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All data generated or analysed during this study are included in this published article and its Additional file 1. So-called virginity testing, also referred to as hymen, two-finger, or per vaginal examination, is the inspection of the female genitalia to assess if the examinee has had or has been habituated to sexual intercourse. This paper is the first systematic review of available evidence on the medical utility of virginity testing by hymen examination and its potential impacts on the examinee. Ten electronic databases and other sources for articles published in English were systematically searched from database inception until January

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Virginity testing: a systematic review

Female virginity is traditionally defined as the state of a woman who has not engaged in penis-vagina sexual intercourse. Female virginity cannot be measured or proven from a physical examination or test. Female virginity is NOT a tangible entity. A hymen is a thin fold or folds of mucous tissue located centimeters inside the vaginal opening that surrounds or may even completely cover the opening. Its color is slightly pink but it can also look whitish.

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